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Unigene blast software
Blast Software, supplied by Unigene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blast+software/blast+software/pmc13065427-108-2-7
Average 86 stars, based on 1 article reviews
blast software - by Bioz Stars, 2026-10
86/100 stars

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Article Title: Transcriptional dynamic changes in energy metabolism, protein synthesis and cell cycle regulation reveal the biological adaptation mechanisms of juvenile Acrossocheilus wenchowensis under acute temperature changes.
Article Snippet: In recent years, frequent acute temperature changes have posed a serious threat to the physiology and survival of fish.. This study utilized RNA-Seq technology to analyze the transcriptional dynamics in the muscle tissues of Acrossocheilus wenchowensis under various acute temperature conditions (16◦C, 20◦C, 24◦C, 28◦C and 32◦C).. Through comprehensive analysis, we identified 11509 differentially expressed genes (DEGs), a gene set (profiles 19) that was significantly up-regulated with increasing temperature, and two weighted gene co-expression network analysis (WGCNA) modules that were significantly correlated with acute temperature changes.

Article Title: Dynamic physiology and transcriptomics revealed the alleviation effect of melatonin on Reaumuria trigyna under continuous alkaline salt stress
Article Snippet: .. Unigene sequences were aligned using BLAST software with several databases, including NR (non-redundant protein database), Swiss-Prot, GO (Gene Ontology), COG (Clusters of Orthologous Groups), KOG (eukaryotic Orthologous Groups), and KEGG (Kyoto Encyclopedia of Genes and Genomes). ..

Article Title: SaTDT enhanced plant tolerance to NaCl stress by modulating the levels of malic acid and citric acid in cells.
Article Snippet: The issue of soil salinization is a global concern that significantly impairs crop productivity, quality, and distribution.. Tonoplast Dicarboxylate Transporter (TDT) is a pivotal malic acid transporter localized on the vacuolar membrane, involving in maintaining intracellular pH homeostasis in plants.. However, the molecular mechanisms and regulatory pathways underlying plant salt tolerance through TDT remain elusive.

Article Title: Study on the tissue-specific distribution of triterpenoid saponins in Panax japonicus var. major based on metabolomics and transcriptomics
Article Snippet: .. Then used BLAST software to compare the Unigene sequence with NR, Swiss-Prot, KOG, Trembl, KEGG, and GO databases; used HMMER software to compare the Unigene amino acid sequence with the Pfam database to obtain Unigene annotation information; and then screened the DEGs. .. The annotation results based on the NR, KOG, KEGG, and GO databases were generated using Metware Cloud, a free online data analysis platform ( https://cloud.metware.cn ).

Article Title: Exogenous auxin-induced rapid modulation of herbaceous peony stem straightness: insights into transient cell wall metabolism responses.
Article Snippet: Key message Exogenous IAA applying within 32 days after herbaceous peony bud germination induces a 6-h transient stem change: first bending then upright.. This process correlates with the degradation of cell wall substances like cellulose and pectin.. Abstract Herbaceous peony (Paeonia lactiflora) holds a growing presence in the cut flower market.

Article Title: Physiological responses and metabolic mechanisms of marine Chlorella under hypotonic stress: Implications for nutrient removal and starch accumulation.
Article Snippet: Tidal estuarine systems are characterized by significant salinity fluctuations and are highly susceptible to nutrient pollution.. Chlorella vulgaris, a marine microalga, shows great potential for nutrient assimilation and biomass production, with a broad tolerance to salinity, making it a promising candidate for ecological restoration in such environments.. However, its physiological and metabolic responses to hypotonic stress in tidal estuaries remain unclear.

Article Title: RNA-Seq uncovers endogenous NO-induced hormone signal transduction and carbon metabolism in response to PEG stress in alfalfa
Article Snippet: .. The unigene functions were annotated based on NR (NCBI non-redundant protein sequences), Swiss-Prot (a manually annotated and reviewed protein sequence database), KOG/COG/eggNOG (Clusters of Orthologous Groups of proteins), GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) using BLAST software [ ]. .. After predicting the amino acid sequences of the unigenes, annotation formation was performed by searching against the Pfam (Protein family) database using HMMER software [ ].

Article Title: The Influence of Sodium Humate on the Biosynthesis and Contents of Flavonoid Constituents in Lemons
Article Snippet: .. Unigene sequences were aligned with the KEGG database using BLAST software (v2.2.31), and KOBAS v2.0 was utilized to acquire KEGG Orthology results for Unigene, providing annotation information. ..

Functional Assay:

Article Title: Transcriptional dynamic changes in energy metabolism, protein synthesis and cell cycle regulation reveal the biological adaptation mechanisms of juvenile Acrossocheilus wenchowensis under acute temperature changes.
Article Snippet: In recent years, frequent acute temperature changes have posed a serious threat to the physiology and survival of fish.. This study utilized RNA-Seq technology to analyze the transcriptional dynamics in the muscle tissues of Acrossocheilus wenchowensis under various acute temperature conditions (16◦C, 20◦C, 24◦C, 28◦C and 32◦C).. Through comprehensive analysis, we identified 11509 differentially expressed genes (DEGs), a gene set (profiles 19) that was significantly up-regulated with increasing temperature, and two weighted gene co-expression network analysis (WGCNA) modules that were significantly correlated with acute temperature changes.

Article Title: SaTDT enhanced plant tolerance to NaCl stress by modulating the levels of malic acid and citric acid in cells.
Article Snippet: The issue of soil salinization is a global concern that significantly impairs crop productivity, quality, and distribution.. Tonoplast Dicarboxylate Transporter (TDT) is a pivotal malic acid transporter localized on the vacuolar membrane, involving in maintaining intracellular pH homeostasis in plants.. However, the molecular mechanisms and regulatory pathways underlying plant salt tolerance through TDT remain elusive.

Sequencing:

Article Title: Study on the tissue-specific distribution of triterpenoid saponins in Panax japonicus var. major based on metabolomics and transcriptomics
Article Snippet: .. Then used BLAST software to compare the Unigene sequence with NR, Swiss-Prot, KOG, Trembl, KEGG, and GO databases; used HMMER software to compare the Unigene amino acid sequence with the Pfam database to obtain Unigene annotation information; and then screened the DEGs. .. The annotation results based on the NR, KOG, KEGG, and GO databases were generated using Metware Cloud, a free online data analysis platform ( https://cloud.metware.cn ).

Article Title: Exogenous auxin-induced rapid modulation of herbaceous peony stem straightness: insights into transient cell wall metabolism responses.
Article Snippet: Key message Exogenous IAA applying within 32 days after herbaceous peony bud germination induces a 6-h transient stem change: first bending then upright.. This process correlates with the degradation of cell wall substances like cellulose and pectin.. Abstract Herbaceous peony (Paeonia lactiflora) holds a growing presence in the cut flower market.

Article Title: Physiological responses and metabolic mechanisms of marine Chlorella under hypotonic stress: Implications for nutrient removal and starch accumulation.
Article Snippet: Tidal estuarine systems are characterized by significant salinity fluctuations and are highly susceptible to nutrient pollution.. Chlorella vulgaris, a marine microalga, shows great potential for nutrient assimilation and biomass production, with a broad tolerance to salinity, making it a promising candidate for ecological restoration in such environments.. However, its physiological and metabolic responses to hypotonic stress in tidal estuaries remain unclear.

Article Title: RNA-Seq uncovers endogenous NO-induced hormone signal transduction and carbon metabolism in response to PEG stress in alfalfa
Article Snippet: .. The unigene functions were annotated based on NR (NCBI non-redundant protein sequences), Swiss-Prot (a manually annotated and reviewed protein sequence database), KOG/COG/eggNOG (Clusters of Orthologous Groups of proteins), GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) using BLAST software [ ]. .. After predicting the amino acid sequences of the unigenes, annotation formation was performed by searching against the Pfam (Protein family) database using HMMER software [ ].



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